动物造模,去势法肾阳虚证动物模型 z-bio 2023-12-20 1446

　　(1) Reproduction method: Adult male rats were anesthetized and bilateral testes were removed. After surgery, a dose of 20000 U/kg of penicillin was injected into the muscle. After 21 days, the stimulation electrode of the electrical stimulator was placed on the penis of the rat, and a voltage of 4mA and 25V was applied to stimulate the penis. The latency period of erection was recorded, and the next day it was weighed. Blood was taken to measure the levels of serum testosterone (T), luteinizing hormone (LH), follicle stimulating hormone (FsH), and cortisol (F). The animal was euthanized and all organs were weighed.

　　(2) The latent period of penile erection induced by animal electrical stimulation is significantly prolonged; The organ index and pituitary organ index of the accessory organs, including the foreskin gland, levator ani muscle, seminal vesicle, and prostate, were significantly reduced; The organ index of the thymus is significantly increased; The organ index of the adrenal gland tends to decrease, while the organ index of the spleen tends to increase. The levels of T and F in animal serum decrease, while the levels of LH and FSH increase.

　　(3) Comparative medicine uses surgery to remove bilateral testicles in male animals or bilateral ovaries in female animals, with the aim of simulating the pathogenesis of kidney deficiency and kidney yang deficiency in traditional Chinese medicine. Male animal castration models are often used for pharmacological or pharmacological studies of aphrodisiac formulas with the main function of improving reproductive function, while female animal castration models are often used for research on kidney deficiency (postmenopausal) osteoporosis and kidney deficiency syndrome. The trauma of castration surgery is significant, and there is a certain gap between the endocrine status after removing the gonads and the simulated clinical decrease in gonadal function.