动物造模,急性肝损伤模型 z-bio 2023-12-20 1926

　　1. Animal modeling materials: BALB/c mice, weighing 20-30g, regardless of gender; Medications: Concanavalin A (ConA), PBS.

　　2. Modeling method: Dilute ConA with PBS and inject 20mg/kg into the tail vein.

　　3. Principle of modeling: Cona bean protein A can activate CD8+or CD4+cell proliferation and increase IFN- γ The release of M promotes the infiltration of inflammatory cells into the hepatic portal vein area and activates M φ The comprehensive response of functional inducing factors produces cytotoxic effects on liver cells.

　　4. Changes after modeling. After 8 hours of injection, serum AST significantly increased; The pathological morphological changes show severe infiltration of neutrophils, lymphocytes, and monocytes in the liver lobules. Inflammatory lesions can also be seen in the portal and central venous areas, and electron microscopy shows diffuse hepatocyte necrosis.