动物造模,离经之血型血瘀证 zi-bio 2024-01-09 1309

　　(1) Reproduction method: Adult rabbits, take 10ml/kg of blood from the heart, inject 20ml into a small beaker, and let it settle. 20 minutes later, the animal underwent a laparotomy under local anesthesia, with the blood clot placed under the colon and sutured to close the abdomen. After 12 days, the autopsy was performed.

　　(2) Model characteristics: After 8 days, the residual amount of blood clots is about 2.1mm3, and after 12 days, it is about 1.8mm3. About 1/4 of the residual blood clots in the abdominal cavity are tightly adhered to the greater omentum or mesentery, while others are in a free state. Microscopic examination revealed unequal amounts of peritoneal wrapping around the blood clot, including free blood clots observed during autopsy. There were piles of macrophages under the peritoneum around the blood clot, which often engulfed red blood cells, hemosiderin, and other cell debris products. Most blood clots had capillaries around them, and eosinophils were found around all blood clots.

　　(3) This model is used in comparative medicine to simulate blood stasis in the abdominal clot of patients with ectopic pregnancy rupture. However, fresh abdominal bleeding in patients is non coagulable or contains flowing blood with clotting patches, while old blood clots are wrapped in hard and thick fibrous connective tissue, firmly adhering to surrounding tissues and organs.