动物造模,气滞血瘀证 zi-bio 2024-01-09 1603

　　(1) Methods Adult rabbits were injected with acupuncture and moxibustion needles subcutaneously in both ears, connected to the electroacupuncture instrument with wires, stimulated with intermittent waves every 10 minutes for 0.5 min each time, causing sudden pain in rabbits, such as screaming, running away, kicking feet and other panic phenomena. The rabbits were stimulated for 6 hours every day, and blood samples were taken from the heart before stimulation, 1 hour, 5 days, 10 days, 15 days and 20 days after stimulation to determine plasma norepinephrine (NE), superoxide dismutase (SOD) The positive percentage of acidic alpha naphthylamine acetate esterase (ANAE) in lymphocytes, microcirculation of eyeballs and conjunctiva, and blood rheology were measured. At the end of the experiment, the animals were euthanized and pathological examination was performed on brain, lung, stomach, tongue, and other tissues.

　　(2) Model characteristics: After 1 hour of pain stimulation, the NE content increased sharply, and animals exhibited strong anti injury reactions such as screaming, running away, and biting wires. As the stimulation time prolonged, the plasma NE content gradually decreased, and the animal's anti injury response gradually weakened; After 5 days of stimulation, SOD activity decreased and free radicals (superoxide anion) in the body increased relatively; The positive percentage of ANAE in lymphocytes slightly increased after 5 days of stimulation and significantly decreased after 10 days. After 5 days of pain stimulation, more than half of the animals had blurred contours of the conjunctival microvasculature, mild dilation of the diameter, uniform reduction of blood flow and gradual appearance of linear granules. The blood color became dark red and even exuded. After 10 days, the changes became more obvious; After 5 days of stimulation, plasma viscosity, whole blood viscosity, and fibrinogen content increased, while the deformability of red blood cells decreased. After 10 days, the above parameters increased to varying degrees, with plasma viscosity and fibrinogen content increasing particularly significantly. After 5 days of pain stimulation, 1/3 of the animals showed partial edema, small blood vessel congestion, purple brown spots, plaques, and other pathological changes on the meninges, lungs, gastric mucosa, and tongue surface. After 10 days of stimulation, more than 2/3 of the animals showed the above changes. Microscopic examination reveals capillary dilation and small venous congestion in the brain parenchyma, gastric mucosa, and lingual interstitium, as well as pulmonary capillary dilation and congestion with scattered bleeding and thrombosis.

　　(3) Compared to medical applications, intermittent sudden pain stimulation in rabbits can indicate extreme anger by continuously kicking after stopping the stimulation. Traditional Chinese medicine believes that anger harms the liver, and anger leads to stagnation of qi. Disordered qi mechanisms can lead to abnormal blood flow. The various manifestations of rabbits after being stimulated by pain fully meet the criteria for judging blood stasis syndrome. The emotional pathogenesis of animals may not be completely consistent with that of humans, and in-depth research is needed to make an objective evaluation.