动物造模,热毒血瘀证 zi-bio 2024-01-09 1523

　　(1) Reproduction method: Adult rabbits were used, and blood samples were taken from the heart before and after the experiment to measure hemorheological and coagulation indicators High viscosity and hypercoagulability model: Blood was taken from the animal's heart, and after 24 hours, a bacterial liquid volume of 2ml/piece (Staphylococcus aureus type A, positive for plasma coagulase, prepared to 0.25 when in use) was used × 1 million live bacteria/ml were slowly injected from the ear vein, and blood was collected from the heart at 24 and 48 hours after the attack Low viscosity and high coagulation model: After 24 hours of heart blood collection, use Escherichia coli endotoxin (LPS) at a rate of 2 μ G/kg body weight was injected from the ear vein, and after 24 hours, LPS was injected again at the same dose. After 0.5 hours, cardiac blood was collected Hypercoagulation model: Animals are given intramuscular injection of dexamethasone injection at a dose of 2mg/kg body weight once a day, and on the 8th day, LPS is used at a dose of 1 μ A dose of g/kg body weight was administered via ear vein injection, and blood was collected from the heart 2 hours later.

　　(2) Model characteristics: After 24 hours of modeling in high viscosity and high coagulation animal models, the whole blood viscosity increased, erythrocyte sedimentation rate increased, and hematocrit decreased. After 48 hours of modeling, the plasma viscosity also significantly increased, erythrocyte sedimentation rate continued to increase, and hematocrit decreased more significantly. The whole blood viscosity decreased to some extent; The whole blood viscosity, plasma viscosity, and hematocrit of low viscosity and high coagulation model animals all significantly decreased, while erythrocyte sedimentation rate significantly increased; The changes in whole blood viscosity, erythrocyte sedimentation rate, and hematocrit in hypercoagulable model animals were not significant, except for a significant increase in plasma viscosity. After 48 hours of modeling in high viscosity and high coagulation model animals, the prothrombin time (PT) was significantly shortened and fibrinogen (Fg) was significantly increased; The PT and partial thromboplastin time (KPTT) of low viscosity and high coagulation model animals were significantly shortened, while fibrinogen decreased; The PT and KPTT of hypercoagulable model animals were significantly shortened, and fibrinogen was significantly increased.

　　(3) The "heat toxin blood stasis syndrome" in comparative medicine is a common pathological change in the development process of various warm heat diseases. Therefore, methods such as Escherichia coli endotoxin, Staphylococcus aureus, and dexamethasone combined with internal toxins were chosen to create an animal model of the "heat toxin blood stasis syndrome". From the appearance of high fever, high viscosity (or low viscosity), high coagulation and other symptoms in animals after modeling, it can basically reflect the clinical characteristics of heat toxicity and blood stasis syndrome.

　　Three different methods were used to create models of "heat toxin blood stasis syndrome", and the similarity in the performance of hemodynamic and coagulation indicators was that after modeling, each model animal showed significant shortening in coagulation indicators such as PT and KPTT; The difference lies in the blood rheological indicators. Animals modeled with Staphylococcus aureus showed a significant increase in whole blood viscosity and plasma viscosity, while those modeled with endotoxins showed a significant decrease. Animals modeled with dexamethasone and endotoxins showed no significant changes.

　　The characteristics of the models replicated by different modeling methods also vary. Using Staphylococcus aureus to create a model can result in sepsis symptoms similar to human infection, with a more obvious phenomenon of "heat toxicity and blood stasis". However, the amount of Staphylococcus aureus invasion in this model is difficult to control. If the dose is too large, animals are prone to death, while if the dose is too small, the lesion is mild, and a model of "heat toxicity and blood stasis syndrome" cannot be replicated. Due to the heavy lesion in this model. It is also difficult to observe the therapeutic effect of traditional Chinese medicine formulas. Endotoxin two