动物造模,高脂血症 z-bio 2024-01-29 1662

　　Method 1

　　1. Animal modeling materials: SD or Wistar rats, regardless of gender, weighing 260-280g; Medication: High fat feed.

　　2. Modeling method

　　(1) Formula 1: 1% -4% cholesterol, 10% lard, 0.2% methylthiouracil or propylthiouracil, 86% -89% basic feed. Continuous feeding for 7-10 days.

　　(2) Formula 2: 10% egg yolk powder, 5% lard, 0.5% bile salt, 85% basic feed, fed for 7 days.

　　3. The principle of modeling is to feed animals with high-fat and high cholesterol feed, which can cause hyperlipidemia in the short term.

　　After 10 days of feeding high-fat feed, the blood lipid levels of rats significantly increased after modeling. Compared with normal rats, blood cholesterol increased by 27.6 times, triglycerides increased by 2.33 times, and low-density lipoprotein increased by 47.8 times.

　　5. Precautions: When the formula is completely consistent, the time for SD rats to establish a model is shorter than that of Wistar rats.

　　Method 2

　　1. Animal modeling materials: Healthy adult SD rats, half male and half female, weighing (180 ± 20) g; Medication: High fat feed (79% base feed, 1% cholesterol, 10% egg yolk powder, and 10% lard).

　　2. Modeling method: Experimental animals were fed with basic feed for observation for 5 days. Tail blood was taken to measure total cholesterol (TC) and triglycerides (TG) as controls, and then fed with high-fat feed for 35 days.

　　3. The principle of modeling is to feed animals with high-fat and high cholesterol feed, which can cause hyperlipidemia in the short term.

　　4. Changes in serum TC and TG levels of high-fat control group rats after 35 days of feeding with high-fat feed were significantly higher than those before feeding with high-fat feed, and the differences were very significant, indicating the successful establishment of a preventive hyperlipidemia rat model.