动物造模,心律失常 z-bio 2024-01-29 657

　　1. Animal modeling materials: Rabbits, weighing around 2kg; Medications: chloroform, adrenaline; Equipment: Biological function experimental system.

　　2. Method of animal modeling: Fix the animal in an upright position, insert the needle electrode into the subcutaneous area of the limbs and connect it to the electrocardiogram and electrocardiogram oscilloscope. Cover the mouth and nose with an anesthesia mask, and slowly drip chloroform onto the mask for inhalation anesthesia. Initially, the rabbit may hold its breath, struggle, and breathe deeply and quickly. At this time, stop inhaling chloroform to prevent large amounts of chloroform from causing poisoning and death. After breathing is stable, continue inhaling chloroform and pay attention to corneal radiation, When the corneal reflex has just disappeared (equivalent to the first level of anesthesia in the third phase), record lead II of the electrocardiogram, immediately stop administering chloroform, and rapidly inject adrenaline 50 through the ear vein μ G/kg, while recording an electrocardiogram, arrhythmia quickly appeared.

　　3. The principle of modeling utilizes adrenaline to induce arrhythmia and establish an animal model.

　　4. After modeling, rapid intravenous injection of adrenaline can lead to one or multiple sources of ventricular premature contractions, paroxysmal tachycardia, and even ventricular fibrillation, lasting for about 3-7 minutes. Most animals fully recover to normal within 10 minutes, while a few animals may experience nodal or ventricular escape, gradually transitioning to nodal or ventricular rhythm, and sinus rhythm can be restored after about 5 minutes.