动物造模,原发性肝癌,药效评价 zi-bio 2024-10-08 812

　　Objective: To establish a good, fast, and stable mouse primary liver cancer model

　　Method: 128 male KM mice were randomly divided into 8 groups: the normal group was given a free diet; The diethylnitrosamine (DEN) aqueous solution group was given a daily aqueous solution containing DEN (16.5 mg/kg); High dose DEN sesame oil group (7 times/week, 3 times/week, 1 time/week), orally administered with sesame oil solution containing DEN (16.5 mg/kg); The low-dose DEN sesame oil group, nano-DEN group, and nanocarrier group were orally administered a sesame oil solution containing DEN (8.25 mg/kg) and nano-DEN or the same amount of nanocarrier each week. After 20 weeks, the mice were euthanized, liver samples were taken, fixed, and HE staining was performed

　　Result: (1) Each group of mice containing DEN showed varying degrees of dry, dull fur, slow movement, and slow weight gain; (2) At 20 weeks, both the high-dose DEN sesame oil group and the DEN aqueous solution group showed tumors, and the mortality rate of mice with one dose per week was lower; (3) The Nano DEN group mice did not die, and their liver damage HE score was more severe than the low-dose DEN sesame oil group

　　Conclusion: Oral administration of DEN dissolved in sesame oil increases the compliance of mice with medication, and long-term low-frequency administration can greatly reduce the mortality rate during the modeling process. The nano microemulsion formulation of DEN can enhance the degree of liver damage