动物造模,性早熟痰湿壅滞证,药效评价 zi-bio 2024-10-11 746

　　Objective: To explore a systematic evaluation method for a rat model of precocious puberty with phlegm dampness obstruction syndrome

　　Method: By observing the syndrome manifestations of female SD rats in the precocious puberty phlegm dampness obstruction group, precocious puberty non phlegm dampness obstruction group, blank control group, parental model group, and parental control group, combined with open field experimental data, this study explores the systematic evaluation method of combining this disease syndrome with animal models

　　Result: There was no significant difference in weight gain between the parental model group and the parental control group (P>0.05). Among the 4-week-old female offspring, the female mice in the precocious phlegm dampness stagnation group showed significant weight loss compared to the blank group (P<0.05), while the female mice in the precocious non phlegm dampness stagnation group showed weight loss compared to the precocious non phlegm dampness stagnation group (P<0.05). The female SD rats in the parental model group showed a significant decrease in total movement distance and movement speed compared to the parental control group (P<0.05), and were significantly higher in the number of stationary times and stationary time than the parental control group (P<0.05). However, there was no significant difference in movement distance, movement speed, stationary times, and stationary time among the three groups of offspring (P<0.05).>0.05)

　　Conclusion: A high-fat diet did not increase the body weight of female SD rats, and the body weight index may not be a key indicator for evaluating the animal model of phlegm dampness stagnation syndrome; The behavioral changes in the rat model of phlegm dampness stagnation syndrome are similar to the clinical manifestations of this syndrome, and further open field experiments can be used to study the evaluation method of the animal model of phlegm dampness stagnation syndrome. No behavioral differences were found among the female offspring groups, and further research is needed on the behavioral aspects of phlegm dampness stagnation syndrome in precocious puberty