动物造模,胃癌转移模型,药效评价 zi-bio 2024-10-21 863

　　Objective: To establish a gastric cancer metastasis model based on clinical tumor specimens and provide personalized animal models for the study of gastric cancer metastasis

　　Method: Fresh surgical specimens of gastric cancer were transplanted subcutaneously into nude mice to establish a patient derived xenograft (PDX) model for gastric cancer patients. The subcutaneous tumor tissue was further transplanted into the gastric muscle layer of nude mice through surgery, and the physical signs of the nude mice were continuously observed. The occurrence of tumor metastasis was detected using near-infrared fluorescence live imaging technology. The tumor bearing mice were dissected, and the lung metastasis was further transplanted subcutaneously into the nude mice to obtain a solid tumor. HE staining was used to observe the structural characteristics of the primary tumor and metastasis, and short tan repeat was used to analyze the genetic characteristics of the primary tumor and metastasis. PCR Array was used to analyze the genetic characteristics of metastasis and primary tumor. The expression of transfer related genes

　　Result: A gastric cancer PDX model was successfully established, and the tissue structure of the transplanted tumor remained basically consistent with that of the patient; Mouse C19751 was found to have lung and liver metastasis through in-situ gastric transplantation. Solid tumors were obtained after subcutaneous transplantation of lung metastases, and STR analysis showed that the primary tumor maintained the same genetic characteristics as lung metastases. PCR Array results showed that compared with the primary tumor, the expression of CXCL1 IGF1 and MMP2 genes was significantly upregulated in metastatic tumors

　　Conclusion: The successful establishment of a gastric cancer metastasis model using clinical tumor specimens provides a good individualized model for the study of gastric cancer metastasis