动物造模,药效评价 z-bio 2024-07-01 297

　　1. Animal modeling materials: healthy rabbits, weighing 1.75-2.15kg; Medications: Anesthetics, pertussis bacterial solution.

　　2. Method of modeling: After anesthesia, the animal is placed in a supine position and a 5cm incision is made at the midline of the neck to expose the right common carotid artery, separate the internal carotid artery, ligate the collateral branch that did not enter the skull, and then clamp the starting point of the right external carotid artery with an arterial clamp. Use a 4.5 gauge needle to inject 0.6ml/kg of pertussis bacterial solution from the right carotid artery, with an injection time limit of about 40 seconds. Then, release the arterial clamp, gently press the needle hole for a few minutes after removing the needle to stop bleeding.

　　3. Modeling principle: Pertussis bacterial solution causes infectious brain edema in animals.

　　4. Changes after modeling: Model animals exhibit symptoms of cerebral edema. The water content of brain tissue was (78.55 ± 0.65)% on the right side and (79.62 ± 0.52)% on the left side in the control group; After 4 hours of injection of bacterial solution, the right side (84.39 ± 1.01)% and left side (80.10 ± 0.81)% were observed in the modeling group.