动物造模,药效评价,心肌缺血 z-bio 2024-07-02 403

　　1. Animal modeling materials: healthy dogs, weighing 10-20kg; Medications: pentobarbital, penicillin; Equipment: airbag compressor, animal artificial respiration machine.

　　2. Method of modeling: After anesthesia, the animal undergoes artificial respiration. After thoracotomy from the 4th or 5th intercostal space, the anterior descending branch of the left coronary artery is separated by about 1cm (or a segment of the left circumflex branch) and placed in a compression device. When the airbag of the compression device expands, it compresses the blood vessels and blocks blood flow, causing local myocardial ischemia for a short period of time. Long term blockage leads to myocardial infarction.

　　Suture 11-15 electrodes on the pericardium or fix a multi-point epicardial electrode, connect a multi-channel physiological recorder, separate the electrode lead and the plastic catheter of the compressor through the chest wall to lead out the body, suture the chest wall layer by layer, and expel the chest gas. After surgery, inject penicillin into the muscle for 3 days, and put a vest on the animal to protect the wound, catheter, and lead. After chest closure, dogs can still block coronary artery blood flow under anesthesia to observe ischemic reactions under anesthesia. After recovery, dogs can observe the pericardial electrogram after coronary artery occlusion under clear and quiet conditions. The duration of each coronary artery occlusion is 3-5 minutes, and the interval time between secondary occlusion is 20-30 minutes. Approximately 2-5 occlusion times per day can be observed continuously for 6-10 days.

　　3. Principle of modeling: The compressor intermittently blocks the coronary artery blood flow in animals, leading to myocardial ischemia.

　　4. Changes after modeling: Changes in animal electrocardiogram after modeling, resulting in myocardial ischemia.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and sterile operations should be strictly carried out.a