动物造模,药效评价,狭窄心血瘀阻证 z-bio 2024-07-29 1094

　　(1) Method of replication: Adult experimental dogs were anesthetized and underwent positive pressure artificial respiration with endotracheal intubation. The fifth rib of the left chest was incised, a heart bag was cut, and a pericardial bed was made. The coronary artery was separated 2cm below the first branch of the left anterior descending (LAD) of the coronary artery, and an electromagnetic flowmeter probe was placed to measure coronary blood flow (CBF). An adjustable micrometer constrictor was placed on the LAD trunk and distal end of the electromagnetic flowmeter probe. The diameter of the constrictor ring was adjusted using a knob to control the diameter of the coronary artery, gradually narrowing the coronary artery and creating a model with a CBF reduction of 70%. Hemodynamic parameters were measured before and after coronary artery constriction, and the stability of each hemodynamic parameter value 30 minutes after constriction was used as the success criterion for modeling.

　　(2) Model features: After coronary artery stenosis, the hemodynamic parameters of left ventricular systolic peak pressure (LVSP), maximum rate of left ventricular pressure increase (+LVdp/dt max), maximum rate of left ventricular pressure decrease (- LV dp/dt max), thoracic aortic pressure (mA0P), CBF, and cardiac output (CO) significantly decrease, while heart rate (HR) remains unchanged.

　　(3) Comparing the animal models of quantitative coronary artery stenosis and blood stasis syndrome in comparative medicine, after coronary artery stenosis, the reduced CBF significantly reduces myocardial blood supply and heart function is impaired, resulting in a decrease in LVSP, CO, etc. After CO reduction, blood flow and velocity decrease, and blood perfusion in various organs decreases. This can be explained by the blood stasis theory of poor blood flow and stasis, which accumulates into blocks. Similarly, this method replicates traditional Chinese medicine syndrome models based on the pathological characteristics of coronary heart disease, which can cause significant trauma to experimental animals. We suggest promoting non-invasive pathogenicity in the replication of traditional Chinese medicine syndrome models.