动物造模,药效评价,葡萄膜炎 z-bio 2024-07-29 508

　　1. Model making method: SD rats, secondary, 6-8 weeks old, weighing 150-180g, male. The endotoxin used was Salmonella typhi endotoxin (LPS, Sigma). The endotoxin was dissolved in sterile physiological saline at a concentration of 2g/L. 50 μ l of endotoxin was injected into the plantar area of the experimental group rats, with a total injection amount of 100 μ g per rat; Perform slit lamp and fundus examination before injection and at 4, 6, 12, 18, and 24 hours after injection. If necessary, use tropicamide to dilate the pupils and record changes in clinical signs in detail. Refer to and modify Hoekzema et al.'s method to evaluate and score clinical manifestations: iris congestion, anterior chamber exudation, anterior chamber pus, and pupil constriction each score 0-2 points, with a total score of 8 points. The normal control group was only injected with 50 μ l of physiological saline solution into the plantar area of both sides.

　　2. Observation indicators and analysis

　　(1) Measurement of aqueous humor protein content: 24 hours after endotoxin injection, an equal amount of ketamine and chlorpromazine (1ml/kg each) were mixed and injected into the abdominal cavity of mice for general anesthesia, followed by local anesthesia with lidocaine eye drops. Under the dissecting microscope, a 29 gauge needle was used to puncture the anterior chamber through the transparent cornea. The aqueous humor of each mouse eye was collected separately and stored in a 20 ℃ refrigerator. The Coomassie Brilliant Blue G2250 dye method was used for quantification of aqueous humor protein. The results showed that the highest concentration of aqueous humor protein in the normal group was 4.2g/L, the lowest was 0.5g/L, and the average was (2.3 ± 1.8) g/L; The highest concentration of aqueous humor protein in the experimental group 24 hours after endotoxin injection was 24.4g/L, the lowest was 18.3g/L, and the average was (21.3 ± 3.0) g/L, with a significant difference between the two (P<0.001).

　　(2) Pathological examination of the eyes: Dilation of iris blood vessels, infiltration of inflammatory cells in the iris ciliary body, anterior and posterior atria, and vitreous cavity, mainly composed of polymorphonuclear neutrophils, with a certain amount of lymphocytes and mononuclear macrophages. A large number of neutrophils adhere to the back of the cornea, thickening of the iris ciliary body stroma, destruction of epithelial cells, loss of normal structure, and pupil occlusion; The retina is significantly thickened, with a large number of polymorphonuclear leukocytes infiltrating, affecting the optic disc. Some areas show subretinal serous exudation, and the choroid is mildly affected, manifested as choroidal edema and inflammatory cell infiltration.