动物造模,药效评价,急性心肌缺血 z-bio 2024-07-29 343

　　1. Modeling material animals: Healthy hybrid dogs, each weighing between 15-20 kg; Medications: Ketamine, Sodium Barbital; Equipment: BL-420 biological function experimental system, animal artificial ventilator.

　　2. The modeling method involves inducing anesthesia with ketamine at a dose of 0.002-0.004g/kg. The dog is fixed in a supine position on the experimental platform, and a venous channel is established. The femoral artery is separated and connected to a fixed blood pressure monitor. The skin is prepared and connected to various leads of the electrocardiogram and a multifunctional monitor. Tracheal intubation is performed, and an anesthesia breathing machine is connected. A mass fraction of 5% barbiturates at a dose of 0.08-0.1g/kg is injected intramuscularly for anesthesia. A midline incision is made to open the chest cavity, cut the pericardium, expose the heart, ligate the second and third branches of the coronary artery, and then close the chest cavity.

　　3. The modeling principle involves ligating the branches of the coronary artery in dogs to establish an acute myocardial ischemia model.

　　4. Changes after modeling: Significant ischemic changes such as S-T segment and T wave were observed on electrocardiograms at 5, 15, and 30 minutes after ligation (10/10); At 45, 60, and 120 minutes, there were still significant changes in myocardial ischemia recorded on electrocardiogram, and the mortality rate was not high, only 10%. The serum creatine kinase MB isoenzyme (CPK-MB) was significantly increased compared to preoperative levels.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.