动物造模,药效评价,戊型肝炎病毒 z-bio 2024-08-02 284

　　Hepatitis E is mainly transmitted through the fecal oral route (digestive tract), while other routes include blood transmission and vertical transmission. By utilizing the transmission characteristics of hepatitis E, animals were induced to develop a disease process similar to that of natural human infection with hepatitis E virus by intravenous injection of fresh fecal filtrate from hepatitis E patients.

　　【 Modeling Method 】 Take acute phase fecal samples from patients with hepatitis E, prepare a 10% suspension in 0.1mol/L PBS buffer, centrifuge and filter the bacteria, and inject rhesus monkeys (Rhesus monkey) via hind limb vein at a dose of 1.5ml/animal.

　　【 Model Characteristics 】 Around the 28th day after infection, ALT begins to increase and reaches its peak within one week. From the 18th to the 28th day, IgG is positive and returns to normal after 120 days. Viremia occurs from the 14th to the 32nd day. On the 7th to 55th day after infection, fecal detoxification was observed, and virus like particles with wavelengths of 27-34nm were observed under electron microscopy in fecal samples. Observation of ultra-thin liver tissue sections under electron microscopy revealed the presence of virus like particles the size of hepatitis E virus in the cytoplasm of the liver, and clusters of virus particles were observed in the fragments of dissolved and necrotic gallbladder epithelial cells. Pathological sections of the liver can observe loose and necrotic liver parenchyma, focal infiltration of a large number of lymphocytes and a small number of neutrophils, destruction of the central vein wall, lymphocyte infiltration, and inflammatory cell infiltration in the portal area.

　　In non-human primate models of hepatitis E, China generally uses rhesus monkeys as animal models. The rhesus monkey hepatitis E animal model can be used for clinical diagnosis, disease prevention, and vaccine development.