动物造模,药效评价,记忆巩固障碍 z-bio 2024-08-05 339

　　1. Modeling material animal: Wistar rats, weighing (216 ± 10) g; Instrument: Electroconvulsive device.

　　2. The modeling method starts with jumping platform training, and immediately after the animal training is completed, electrodes are connected to the head and nose to apply current. Perform maximum electroconvulsive therapy with a stimulation intensity of 150V, a current of 0.1A, and a duration of 0.3s. Afterwards, limb rigidity will be used as the shock indicator for 30 minutes, followed by memory measurement.

　　3. Modeling principle: Electric shock leads to memory consolidation disorders.

　　4. After modeling, the water maze test is usually conducted 24 hours later. The animal's searching platform latency (SPL) is (20 ± 8) seconds before electroconvulsion, (55 ± 17) seconds 30 minutes after electroconvulsion, and (29 ± 10) seconds after 24 hours; Within 120 seconds after the removal of the underwater platform, the percentage of times animals cross the corresponding positions of the original underwater platform to the total number of times they cross the corresponding positions of each quadrant platform (crossing platform percentages, CPP) was (58 ± 4)% before electroconvulsion, (27 ± 5)% 30 minutes after electroconvulsion, and (34 ± 3)% 24 hours later. The results showed that after electroconvulsive therapy in the model rats, SPL was prolonged compared to before shock, CPP decreased, and varying degrees of memory loss and spatial localization ability were observed.