动物造模,药效评价,烟曲霉菌感染 z-bio 2024-08-19 865

　　(1) Modeling method: New Zealand white rabbits were injected with 440mg/square meter of cytarabine via ear vein daily from day 1 to day 5 of the experiment, and maintained in a low immune state every other day after 6 days. Starting from the 4th day, both groups were given intravenous injections of vancomycin 15mg/kg and cefotaxime 150mg/kg once daily; Gentamicin 5mg/kg intravenous injection, once every other day. On the second day of the experiment, endotracheal inoculation was performed, and after anesthesia, 0.4ml (concentration 5 × 10 ^ 7/ml) of Aspergillus solution was injected into the trachea. Control group: Inject 0.4ml of physiological saline into the trachea. All surviving New Zealand white rabbits were subjected to lung CT scans on day 1 before vaccination, day 2, day 4, day 6, day 8, day 10, day 12, day 14, day 16, and day 18 after vaccination. And after each CT scan, two experimental rabbits with abnormalities were euthanized and specimens were collected for pathology and fungal culture. At the end of the experiment, collect pathological and fungal culture results, and summarize all CT findings.

　　(2) Result: 17 New Zealand white rabbits were successfully inoculated with Aspergillus fumigatus. CT scans showed lung abnormalities in 6 rabbits on the second day of inoculation, 4 rabbits on the fourth day, 3 rabbits on the sixth day, 2 rabbits on the eighth day, 1 rabbit on the twelfth day, and 1 rabbit on the eighteenth day. During the experiment, 10 experimental rabbits died spontaneously. The pathological changes manifest as pulmonary congestion, bleeding, interstitial proliferation, infiltration of inflammatory cells, tissue necrosis, and abscess formation.