铅性肾病,动物造模,药效评价 z-bio 2024-08-26 372

　　1. Modeling material animal: Healthy male SD rats, weighing (220 ± 20) g; Drug: Lead acetate.

　　2. Modeling method control group: distilled water gavage, 1 hour later, intraperitoneal injection of physiological saline, 5 times a week, for a total of 12 weeks; Model group: Intraperitoneal injection of 0.5% lead acetate solution (5mg/kg), 5 times a week, for a total of 12 weeks.

　　3. Modeling principle: Lead poisoning can cause kidney damage.

　　4. Compared with the control group, the animal model group showed a general change after modeling. Rats in the animal model group had a decrease in body weight after the second week of exposure, and a significant decrease in body weight after the third week. In addition, there is a decrease in activity, fluffy back hair, and a lack of luster. A few rats are covered in filth.

　　During the experiment of biochemical and pathological changes after modeling, the urine protein content of the exposed group continued to increase, and by the 12th week, the urine protein content was about 15 times higher than before exposure. There was no significant change in glomerular filtration rate.

　　Lead exposure significantly increased urinary gamma glutamyl transpeptidase (γ - GT) activity, urinary delta amino gamma ketovaleric acid (δ - ALA) content, and blood zinc protoporphyrin (ZPP) values in the model group at all time points compared to the normal control group.

　　The urinary lead excretion and renal lead content in the exposed group were significantly higher than those in the control group at all time points.

　　Observation results under light microscope: No significant abnormalities were observed in the control group. In the 6th week, the model group showed congestion of glomerular capillaries and degeneration of epithelial cells in the renal proximal tubules. The changes became more pronounced in the 12th week, with extensive vacuolar degeneration and focal necrosis. Mild vacuolar degeneration was also observed in the distal tubules.

　　Electron microscopy observation results: visible nuclear condensation, mitochondrial swelling, and blurred cristae.