动物造模,药效评价,性胃溃疡 z-bio 2024-08-26 403

　　(1) Method of replication: Adult rats were fasted without water for 24 hours, anesthetized with pentobarbital sodium, and opened in a supine position through a midline incision. The lateral wall of the jejunum, 10cm away from the Treitz ligament, was anastomosed with the anterior wall of the anterior stomach in a peristaltic direction. After surgery, the rats were fasted without water for 3 days; Four weeks after gastrointestinal anastomosis. Open the abdomen again and cut off the distal duodenum at the opening of the biliary pancreatic duct, embedding both ends separately. Two weeks after reflux surgery, anesthesia was applied to open the abdomen. Fasting gastric juice was aspirated from the anterior wall of the anterior stomach, centrifuged, and the pH value of the supernatant was measured; Make a small incision on the anterior wall of the glandular stomach, insert the detection electrode in the direction of the small and large bends, and measure the blood flow of the glandular stomach mucosa in these two parts; Collect blood from the inferior vena cava, centrifuge, prepare serum, and analyze serum gastrin levels; Extract the entire stomach, cut it along the greater curvature, observe mucosal lesions, and parallel cut the gastric antrum lesser curvature and glandular anterior wall tissue along the lesser curvature line for histological examination, while measuring mucosal thickness.

　　(2) Model characteristics: The pH value of gastric juice in the model animals increases, the blood flow of gastric mucosa decreases, and the serum gastrin level increases. Pathological examination showed multiple circular or elliptical ulcers on the lesser curvature side of the gastric antrum, with neat edges and necrotic exudate attached to the ulcer base. The non ulcer mucosa was edematous and thickened, and the mucosa in the pyloric gland area and gastric fundus gland area was thickened. The ulcer area on the lesser curvature side of the gastric antrum was (7.4 ± 2.9) square millimeters, and the thickness of the mucosa in the pyloric gland area and gastric fundus gland area increased by 29% and 35%, respectively.

　　(3) The reflux of duodenal contents into the stomach in comparative medicine can cause inflammation and damage to the gastric mucosa, and damaged gastric mucosa is more susceptible to acid and pepsin damage. Based on the mechanism of duodenal contents damaging the gastric mucosa, this experiment used surgical methods to continuously reflux all duodenal contents into the stomach of rats, creating a typical model of gastric antral small curvature ulcer. Its lesions are more in line with the common site and morphological characteristics of human gastric ulcers, and histology shows that this ulcer is very similar to the pathological changes of human gastric ulcers. Pathophysiology is more in line with the natural development process of gastric ulcer formation, making it a commonly used experimental animal model in the field of gastric ulcer research.