动物造模,药效评价,抑郁症 z-bio 2024-08-30 628

　　1、 The antagonistic effect of drugs

　　1. Lysine antagonism is the earliest animal model established for antidepressant research after the discovery that monoamine oxidase inhibitors and tricyclic antidepressants can counteract the abnormal behavior caused by Lysine. It is commonly used for the initial screening of antidepressants with certain pharmacological effects.

　　2. Antagonism of high-dose apomorphine. This model reflects the drug's effect on norepinephrine (NA) reuptake or NA delivery, and can be used to screen antidepressants that inhibit NA reuptake or activate α 1-adrenergic receptors and β 2-adrenergic receptors. Currently, it is only used for initial screening of antidepressants. And it is rarely used alone.

　　2、 Induction effect of drugs

　　The effect of 1.5 hydroxytryptamine induced head shaking behavior antidepressants is to enhance central NA and/or 5-hydroxytryptamine (5-HT) function. Some antidepressants can enhance the action of 5-HT by blocking its reuptake. Serotonin is a precursor of 5-HT, and administration of monoamine oxidase inhibitors (MAOIs) can inhibit metabolism, followed by antidepressants to enhance its typical symptom - head shaking behavior. This model is commonly used to study and screen antidepressants that act on the central 5-HT or NA system.

　　2. Chlormipramine induced model animals. The application of Chlormipramine can cause behavioral defects and rapid eye movement (REM) sleep abnormalities in adult animals, as well as reduced sexual behavior in male mice. Studies have confirmed that this model animal has abnormal conduction of the 5-HT and noradrenergic systems in the brain, which can be used for research on the monoamine neurotransmitter hypothesis of depression.

　　3. The induction model of bipolar depression and mania by ouabain is related to the decreased activity of Na+- K+- ATPase membrane ion pumps. The Na+- K+- ATPase inhibitor ouabain can induce changes in depressive behavior in SD rats, and this model can be used for the study of bipolar depression.

　　3、 Other effects of drugs

　　1. The mouse Yuhengbin enhanced model can enhance the lethal effect of Yuhengbin on mice when combined with antidepressants that inhibit NA inactivation or NA reuptake. This model is mainly used to observe antidepressants that inhibit NA reuptake.

　　2. Continuous injection of glucocorticoids in the glucocorticoid model resulted in weight loss in rats. Discovered through FST testing. The immobility time significantly increased while the swimming time decreased, but did not change the latency period of struggle time and immobility time. There were no anxiety like or depression like behavioral characteristics in the open field test. This model is still based on stress theory, by artificially increasing the levels of glucocorticoids in the body. Simulating behavior induced biological changes greatly shortens the biological process of modeling, thus shortening the modeling cycle. This model has the potential to become a novel and effective animal model for depression.