动物造模,药效评价,急性肾衰犬 z-bio 2024-09-19 467

　　1. Modeling material animals: 20 healthy adult beagle dogs weighing 13-20kg; Reagent: Artificial seawater [Main indicators: osmotic pressure (1250 ± 11.52) mmol/L, pH 8.2, sodium ion (630 ± 5.33) mmol/L, potassium ion (10.88 ± 0.68) mmol/L, chloride ion (658.8 ± 5.25) mmol/L, seawater temperature 19-21 ℃, laboratory temperature 25 ℃], ketamine, rapid sedative, diazepam.

　　2. Modeling method: Animals were fasted for 12 hours before surgery, anesthetized with intramuscular injection of ketamine (20mg/kg), and maintained with a mixture of ketamine and diazepam injection. The animal is lying on its back, with its limbs fixed, and skin preparation is performed in the surgical area of the neck and abdomen. Insert a double lumen catheter through the left carotid artery and jugular vein to measure blood pressure and prepare blood samples. Injury: An incision is made in the middle of the lower xiphoid process of the abdomen, measuring 8cm in length and reaching a depth of the entire abdomen, with visible abdominal organs. After the injury, the wound site was hemostatic. The animals in the seawater immersion group were fixed on a dog mount and immersed in 100L of artificial seawater at a temperature of about 19-21 ℃ for 1.5 hours, with the immersion plane reaching 5cm above the xiphoid process. The control group dogs were not immersed in seawater after suffering from open abdominal injuries.

　　3. The simple principle of modeling is that an open abdominal injury combined with seawater immersion can cause acute kidney failure in animals.

　　4. Changes after modeling: In the seawater immersion group, 2 animals died within 12 hours after injury and all died within 16 hours, while the control group did not die within 16 hours.

　　Vital signs, intra group comparison: Both groups of animals showed a decrease in anal temperature and mean arterial pressure, while the soaking group showed a significant decrease in both indicators after soaking. Inter group comparison: The low temperature and hypotension in the soaking group were significantly worse than those in the control group at various time points after soaking.

　　Blood electrolytes, intra group comparison: The control group animals showed mild increases in osmotic pressure and blood sodium and chlorine levels, with no statistically significant data at most time points; The soaking group animals showed significant increases in all three items. Inter group comparison: The soaking group had significantly higher data than the control group at all time points after soaking.

　　Blood gas analysis, intra group comparison: The pH and HCO3- levels of the two groups of animals showed a significant decrease compared to before injury, indicating an acidic state. Inter group comparison: The degree of acid substitution in the soaking group was significantly higher than that in the control group at various time points after injury.

　　Blood creatinine and urea nitrogen, intra group comparison: Both groups of animals gradually increased urea nitrogen after injury. The creatinine level in the soaking group increased significantly, while there was no significant trend of creatinine change in the control group. Inter group comparison: The soaking group showed a greater increase than the control group, with statistical significance at 8 hours. The creatinine level in the soaking group was significantly higher than that in the control group at 12 hours.

　　The appearance color of the kidneys in the soaking group is dark red. Under light microscopy, the glomerular capillaries, cortical and medullary blood vessels are significantly dilated and congested, with a large amount of inflammatory cell infiltration and microthrombi visible. Translucent degeneration and vacuolar degeneration can be seen in some of the proximal tubule cells. The epithelium of the distal tubules sheds and disappears, and a large number of protein casts can be seen in the lumen. Renal interstitial vascular congestion and edema. The control group showed congestion, cell degeneration, and inflammatory cell aggregation in the kidneys, but significantly milder than the soaking group. No microthrombi were observed.