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How to judge the sex of experimental animals?

How to judge the sex of experimental animals?

Experimental animals of different genders have different responses to many experimental methods, drug sensitivity, and responsiveness to various stimuli. Therefore, animal gender is usually an important part of animal selection....

2020-10-15
What relevant preparations should be made before animal experiments?

What relevant preparations should be made before animal experiments?

What relevant preparations should be made before animal experiments?...

2020-10-15
What is fetal vascular perfusion in miniature pigs?

What is fetal vascular perfusion in miniature pigs?

Continuous infusion of lead oxide particles into 19 fetal pigs at different developmental stages, the pressure is less than 30 mmHg, the fetal blood vessel structure has always been powerless. This is the turning point of fetal malformations and may ...

2020-10-15
What is minipig vesicoureteral reflux?

What is minipig vesicoureteral reflux?

Urinary bladder reflux (VUR) refers to the abnormal physiological phenomenon of bladder urine reflux to the urethra, renal pelvis and limbs caused by multiple primary or secondary causes...

2020-10-15
How to effectively care for small pigs during perioperative period?

How to effectively care for small pigs during perioperative period?

Purpose of intravenous infusion The purpose of hydration during anesthesia is to maintain open venous access, provide circulatory support for oxygen supply, replenish perceptible fluid loss, replenish imperceptible fluid loss, and perform infusion. I...

2020-10-15
How to anesthetize small pigs?

How to anesthetize small pigs?

Fasting before anesthesia. Miniature pigs should be observed for 5-7 days before anesthesia. Dehydration and weight loss may be caused during transportation. Standard care procedures should be established for newly introduced miniature pigs....

2020-10-15
How to prepare a small pig deaf animal model?

How to prepare a small pig deaf animal model?

The species difference between minipigs and humans is very small. They are relatively close to humans in terms of organ structure and metabolism. Many diseases share common causes with humans. It is an ideal animal model for studying various human di...

2020-10-15
How to cultivate minipig parotid gland cells?

How to cultivate minipig parotid gland cells?

4-month-old experimental mini-pigs were subjected to conventional anesthesia. Under aseptic conditions, the parotid alveolar tissue was extracted, the glandular lobule envelope and other binding tissues were removed, and then immersed in HamF12 mediu...

2020-10-15
How to grow periodontal ligament cells of miniature pigs?

How to grow periodontal ligament cells of miniature pigs?

A 6-month-old miniature pig weighs about 25 kg. Miniature pigs will be anesthetized regularly. Pull out the eight anterior teeth (4 dogs and 4 incisions) of the upper and lower jaws of the miniature pig under aseptic conditions, and immediately put t...

2020-10-15
How to cultivate miniature pig dental pulp stem cells?

How to cultivate miniature pig dental pulp stem cells?

How to cultivate miniature pig dental pulp stem cells?...

2020-10-15
What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?...

2020-10-14
How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?...

2020-10-14
How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?...

2020-10-14
What is the method of genotyping?

What is the method of genotyping?

Genotype (genetic type) refers to the type of genes related to the traits contained in the DNA of an organism...

2020-10-14
What is embryonic stem cell method?

What is embryonic stem cell method?

The Martin Evans Institute has successfully cultivated a pluripotent stem cell line from the internal cell mass of early embryos, which can grow in vitro and can differentiate into other cells even if it is reinjected into germ cysts , These cells (i...

2020-10-14
What is the retroviral vector infection method?

What is the retroviral vector infection method?

Studies have shown that retroviruses, especially lentiviral vectors, can effectively introduce foreign DNA into egg cells...

2020-10-14