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【Animal modeling】-IVC cage changing standard operating procedures and the risk of transmission of mouse virus pathogens

【Animal modeling】-IVC cage changing standard operating procedures and the risk of transmission of mouse virus pathogens

To study the risk of virus infection in mice raised with IVC equipment in the standard operating procedure of cage changing in independent ventilation cages and laboratory animal negative pressure barrier facilities...

2022-03-07
【Animal modeling】-Gene cloning and preliminary functional study of junctional adhesion molecule A in tree ferrets

【Animal modeling】-Gene cloning and preliminary functional study of junctional adhesion molecule A in tree ferrets

To obtain the full-length coding sequence of junctional adhesion molecule A in tree ferrets and analyze its molecular characteristics, and to explore the mechanism of JAM-A as a receptor for reovirus invading primary lung cells of tree ferrets...

2022-03-07
【Animal modeling】-Establishment and application of TaqMan real-time quantitative PCR detection method for VP2 gene of mink Aleutian virus

【Animal modeling】-Establishment and application of TaqMan real-time quantitative PCR detection method for VP2 gene of mink Aleutian virus

To establish a convenient and sensitive fluorescent quantitative PCR method for detection of mink Aleutian virus (AMDV), so as to realize the rapid quantitative detection of AMDV in clinical samples...

2022-03-07
【Animal Modeling】-Small ubiquitin-like modifier protease 3 regulates autophagy in mouse alveolar type II epithelial cells

【Animal Modeling】-Small ubiquitin-like modifier protease 3 regulates autophagy in mouse alveolar type II epithelial cells

To investigate the regulatory effect and molecular mechanism of small ubiquitin-like modifier-specific protease 3 on autophagy in mouse lung tissue...

2022-03-04
【Animal modeling】-Validation of the effect of SPF golden hamster cage and drinking water pulsed vacuum sterilization

【Animal modeling】-Validation of the effect of SPF golden hamster cage and drinking water pulsed vacuum sterilization

To confirm the performance of the pulse vacuum sterilizer, and to verify the effect of pulse vacuum sterilization on the SPF golden hamster cage and drinking water under the specified loading method and sterilization conditions...

2022-03-04
【Animal Modeling】-Isolation, Culture and Identification of Mouse Lung Mesenchymal Stem Cells

【Animal Modeling】-Isolation, Culture and Identification of Mouse Lung Mesenchymal Stem Cells

To establish a stable technique for the isolation, culture and identification of mouse lung mesenchymal stem cells, and to study the mechanism of lung tissue repair and regeneration using mouse lung mesenchymal stem cells...

2022-03-04
【Animal Modeling】-Construction of TauT Gene Knockout Rats in the Central Nervous System and Its Effects on Mitochondrial DNA Oxidative Damage

【Animal Modeling】-Construction of TauT Gene Knockout Rats in the Central Nervous System and Its Effects on Mitochondrial DNA Oxidative Damage

To construct a conditional knockout rat of taurine transporter in the central nervous system by combining CRISPR/Cas9 and Cre-loxP technology, and to conduct preliminary research on mitochondrial mtDNA and mitochondrial respiratory chain enzyme activ...

2022-03-04
【Animal Modeling】-Comparative Study on Fine Behavior of Mouse Spinal Cord Injury Strike Model

【Animal Modeling】-Comparative Study on Fine Behavior of Mouse Spinal Cord Injury Strike Model

To explore the abnormal performance of fine behaviors in homecage such as autonomous movement, exploratory behavior and self-care behavior in mice with spinal cord injury in addition to motor dysfunction...

2022-03-04
【Animal modeling】-CD137 signaling regulates endothelial cell-mesenchymal transition and promotes cardio-cerebral angiogenesis in mice

【Animal modeling】-CD137 signaling regulates endothelial cell-mesenchymal transition and promotes cardio-cerebral angiogenesis in mice

To investigate whether CD137-CD137L signal (CD137 signal for short) promotes cardio-cerebral angiogenesis by regulating endothelial cell-mesenchymal transition...

2022-03-04
【Animal Modeling】-Discussion on the role of pathogenic microorganism prevention and control in laboratory animal facility management and biosafety control

【Animal Modeling】-Discussion on the role of pathogenic microorganism prevention and control in laboratory animal facility management and biosafety control

Biosafety management is an important part of laboratory animal facility management...

2022-03-04
【Animal Modeling】-Effect of ambient air exposure on bacterial flora and metabolites in pig manure suspension

【Animal Modeling】-Effect of ambient air exposure on bacterial flora and metabolites in pig manure suspension

To study the effects of ambient air exposure on microorganisms and metabolites in prepared fecal suspensions...

2022-03-04
【Animal modeling】-Quantitative analysis of liver tumors in BALB/c mutant curly mice based on Image J software

【Animal modeling】-Quantitative analysis of liver tumors in BALB/c mutant curly mice based on Image J software

To investigate the differences between BALB/c mutant curly-haired mice and normal BALB/c mice to construct tumor models via portal vein injection, and to establish a quantitative analysis method of tumor images based on Image J software...

2022-03-04
【Animal modeling】-Analysis of genetic quality of Wistar rat microsatellite DNA population based on group standard T/CALAS 21—2017

【Animal modeling】-Analysis of genetic quality of Wistar rat microsatellite DNA population based on group standard T/CALAS 21—2017

To analyze the genetic quality of Wistar rats in closed groups at different periods by using the method recommended by the group standard T/CALAS 21-2017, and to evaluate the applicability of the group standard T/CALAS 21-2017...

2022-03-04
【Animal modeling】-Dynamic changes of myocardial fibrosis time series in spontaneously hypertensive rats

【Animal modeling】-Dynamic changes of myocardial fibrosis time series in spontaneously hypertensive rats

To observe the time-series dynamic changes of myocardial fibrosis in spontaneously hypertensive rats...

2022-03-04
【Animal Modeling】-Analysis of Intestinal Microorganisms in Miniature Pig Type 2 Diabetes Model

【Animal Modeling】-Analysis of Intestinal Microorganisms in Miniature Pig Type 2 Diabetes Model

Recent studies have shown that gut microbes are related to metabolic diseases such as type 2 diabetes mellitus (T2DM), and related mechanisms are mostly studied in rodents, but there are few reports on pig models that are more suitable for studying h...

2022-03-04
【Animal Modeling】-Weinong shows that miRNA can be used as a predictor of mastitis in dairy cows

【Animal Modeling】-Weinong shows that miRNA can be used as a predictor of mastitis in dairy cows

Bovine mastitis is the most common disease in dairy cows and the most expensive disease to treat...

2022-03-04