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Add 1 ml ovalbumin phosphate buffered saline (PBS) (100μg ovalbumin, 10-20 mg aluminum potassium sulfate, pH 7.4 (including) intraperitoneal injection to rats or guinea pigs...
2021-03-29Replication method Experimental animals are usually selected from guinea pigs (8-10 weeks old) and rats (6-8 weeks old). Excessive carbon dioxide kills the guinea pigs and inhales the central cornea of the guinea pigs, and then splits them with guine...
2021-03-29Replication method Anesthetize healthy New Zealand rabbits weighing 2.0 to 3.0 kg by intramuscular injection of 15% bufenazone at a body weight of 20 mg/kg...
2021-03-29Copy method 4-5 kg rhesus monkey, diameter 8 mm, -10.00D surface corneal lens is made of human corneal material, other corneal materials are flat lenses or directly use the anterior corneal layer as an implant...
2021-03-29Replication method (1) Choose female Balb/c mice (body weight 14 to 16 g) aged 4 to 6 weeks, and inject pentobarbital sodium intraperitoneally at a dose of 45 mg/kg body weight... Next, anesthetize, Place it under a microscope, scratch the corneal ep...
2021-03-29Prepare sustained-release endotoxin polymer pellets by the replication method: wash the ethylene-vinyl acetate polymer beads with high-purity hexanal to spectrophotometric purity, and dissolve the polymer in methyl chloride at room temperature to 10%...
2021-03-29Reproduction method When using copper chloramine hydrochloride (50 mg/kg body weight) and chlorpromazine (10 mg/kg body weight), New Zealand rabbits weighing 2.5 to 3.5 kg and weighing more than 4.5 kg are unlikely to induce corneal neovascularizatio...
2021-03-29Breeding method 30 mg/kg body weight of pentobarbital sodium was injected intraperitoneally into male Wistar rats weighing 200 to 220 g, and fixed on the operating table in supine position...
2021-03-26Breeding method Inject cyclophosphamide solution into a New Zealand female rabbit weighing about 1.5 kg at a dose of 8.0×10-2 g/kg from a guinea pig intravenously. After 6 hours, use 0.1, (Xinjieerjie) to treat the rabbit near the vulva of the rabbit...
2021-03-26Radical nephrectomy (1) Replication method An adult rat weighing about 250 g is anesthetized by intraperitoneal injection of 30 mg/kg body weight of pentobarbital sodium...
2021-03-26Replication methods ① Kanamycin model: The rats were deprived of water for 24 hours before the experiment, and then intramuscularly injected with kanamycin at a dose of 200 mg/kg body weight. After 24 hours, blood was collected to measure BUN and SCr...
2021-03-26After renal ischemia/reperfusion, the content of SOD in the kidney tissue of model rats decreased, and the content of MDA increased significantly, and the content of both showed more obvious gradual changes as the reperfusion time continued....
2021-03-26Reproductive methods Gentamicin (GM) was injected subcutaneously into adult rats weighing 200 to 260 g twice a day for 2 consecutive days at a weight of 400 mg/kg. Rat urine was collected on days 2, 5, 8, 11, 15, 15, 22, and 29 after the first inject...
2021-03-26Breeding method The anesthesia method of sodium pentobarbital is to inject 30 mg/kg of body weight intravenously into New Zealand rabbits weighing 2 to 3 kg, fix them on the back operating table, and regularly disinfect the abdominal skin...
2021-03-26Adult rats weighing 250～300g are anesthetized by intraperitoneal injection of 5% chloral hydrate. After anesthesia, they are placed in the supine position and fixed on the operating board....
2021-03-26Rats weighing about 200g, prohibit drinking water for 24 hours, and tie the penis of the rat with a thin thread in advance. Under ether inhalation anesthesia, the animal was placed on the back and fixed on the surgical board. The abdominal skin was r...
2021-03-26