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The replication method is to select an experimental dog and inject anesthetized ketamine hydrochloride intramuscularly at a dose of 30 to 50 mg/kg body weight. After placing the animal under general anesthesia, fix it on the back, make a central inci...
2020-08-08Breeding method The rat weighs about 200 g and is kept in a clean environment. Every morning and afternoon, 15% ammonia water is sprayed on the animal’s pharynx with a slaughter spray, 3 times every 3 days...
2020-08-08Replication method Anesthetize 2.5 to 3.5 kg New Zealand rabbits by intraperitoneal injection of 5 ml/kg of urethane, and fix them on the back operating table...
2020-08-08Breeding method Collect type 3 capsular pneumococcus, inoculate twice to enhance the pathogenicity of mice after live bacteria inoculation, and adjust its concentration to 120×10 million CPU/ml with sterile normal saline...
2020-08-08Breeding method: New Zealand rabbits weighing 2.0-2.5 kg were injected intramuscularly with ketamine hydrochloride at a weight of 50 mg/kg. After anesthesia and fixation, the midline of the rabbit\'s nose was regularly disinfected, depilated, and sus...
2020-08-08Directly inject bacteria into the nasal cavity: use adult male rats weighing 200-250g and use a syringe to directly inject a certain amount of bacteria into the rat\'s sinus cavity without blocking the sinus opening after injection. According to expe...
2020-08-08Reproduction method In adult male rats weighing 200 to 250 g, 1 mg ovalbumin (EA) and aluminum hydroxide gel are used as adjuvants, injected intraperitoneally, and 100 mg pertussis toxin is collected. Intraperitoneal injection, repeat injection after...
2020-08-08Method of reproduction: Inject sodium pentobarbital (35 mg/kg body weight) intraperitoneally into 400-450 g pure white red-eyed guinea pigs for anesthesia, place the head on a special head frame and insert it into the trachea...
2020-08-08Reproduction method When the rat weighs about 200 g and the guinea pig weighs about 500 g, the external auditory canal is clean, the eardrum is intact, there is no spontaneous nystagmus, and the hearing is sharp....
2020-08-08Replication method 4-week-old inbred male C57BL/6 mice and guinea pigs weighing 300 to 400 g exhibited normal ear reflex and no middle ear infection. After the guinea pig is anesthetized, the heart is perfused, the temporal bone is decapitated, all i...
2020-08-08A variety of healthy general-grade guinea pigs, male or female, weighing 400-500 g, sensitive to ear reflexes, and otoscope examination ruled out inflammation of the outer and middle ear...
2020-08-08The replication method uses 300-420 g guinea pigs and reflects players normally. First, perform an otoscope examination to check for external and middle ear diseases. Exclude and test separately...
2020-08-08Breeding method Anesthetize adult cats weighing 2.0 to 3.0 kg by intramuscular injection of 20 mg/kg body weight of ketamine and 2.5 mg/kg disi epa...
2020-08-08C57BL/6N mice born 7 days after birth were placed in a 75% oxygen environment, kept at room temperature (23±2) ℃, and returned to the air environment after 5 days...
2020-08-08Copy method Anesthetize 8-10 kg piglets by intramuscular injection of 0.1 ml/kg of compound ketamine, use compound troponin to dilate pupils and fix the head on the head...
2020-08-08Replication method (1) Culture of rabbit skin fibroblasts: Cut a New Zealand rabbit weighing about 2 kg into 1 piece, disinfect the skin with 75% ethanol, and use cornea quhuaning to cut into the dermis to a diameter of 8 mm incision...
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