【Animal modeling】-Directed editing of mouse MAD2L1 gene based on CRISPR/Cas9 system and analysis of its off-target effect

To establish a method system for gene editing of the second exon of mouse MAD2L1 gene using CRISPR/Cas9 system, and to analyze the off-target effect of CRISPR/Cas9 on MAD2L1 gene editing...

2024-02-22

[Animal modeling] - 3D reconstruction of lungs and 3D printing model of vessels in miniature pigs

To explore the method of 3D reconstruction model of miniature pig lung and the application of 3D printing model...

2024-02-22

【Animal Modeling】-Possible Mechanism of Exogenous β-NGF in Regulating the Process of Bone Remodeling

To explore the possible mechanism of exogenous β-NGF on bone remodeling during bone defect healing...

2024-02-22

[Animal modeling] - Effect of long-term high protein diet on liver steatosis and fat accumulation in OVX mice

To investigate the effects of long-term high protein diet on liver and adipose tissue of ovariectomized mice and the role of glucagon like peptide-1...

2024-02-22

【Animal modeling】-Analysis of the effect of portal hypertension on serum endotoxin and intestinal mucosa in rats

To study the effect of simple portal hypertension on serum endotoxin and intestinal mucosa in rats?...

2024-02-22

[Animal modeling] - Differences in the effects of dietary fat on physiological and biochemical indicators of mice in different feed models

To explore the differences of physiological and biochemical indexes of different oils in mice under different feed models, and to provide experimental basis for the selection and evaluation of feed oils in animal models and the use of oils for human...

2024-02-22

[Animal modeling] Anesthetic effect of compound doses of Shutai and Lumianning on Bama miniature pigs

To study the anesthetic effect of Shutai and Lumianning combined anesthesia on Bama mini pigs, and to observe the effect of anesthesia on physiological indexes of mini pigs...

2024-02-21

【Animal modeling】-Detection and analysis of multiple cytokines in serum of rhesus monkeys and cynomolgus monkeys

To determine and compare basal serum cytokine levels in healthy rhesus monkeys and cynomolgus monkeys...

2024-02-21

[Animal Modeling] - Establishment and Evaluation of a Constipated Irritable Bowel Syndrome Model with Liver Depression Syndrome

o prepare rat models of liver depression syndrome constipation type irritable bowel syndrome (IBS-C) using single factor modeling method and multi factor combined modeling method, and to provide an ideal IBS-C experimental animal model by comparing d...

2024-02-19

[Animal modeling] - Effect and mechanism of androgen on mitochondrial function of gastric mucosa in aged rats

To investigate the effect of androgen on mitochondrial function of gastric mucosa in aged rats with testicular resection (GDX) and its mechanism...

2024-02-19

[Animal modeling] - establishment and evaluation of a rat model of coronary microvascular disease with Qi deficiency and blood stasis syndrome

The incidence rate of coronary microvascular disease is increasing year by year. Traditional Chinese medicine believes that CMVD belongs to the category of \"collaterals\" in traditional Chinese medicine, and the syndrome of qi deficiency a...

2024-02-19

Research progress on diet induced non-alcoholic fatty liver disease animal models

Establishing scientifically effective animal models is a crucial step in completing disease research. Dietary induction is a common method for establishing animal models of non-alcoholic fatty liver disease...

2024-02-19

[Animal modeling]- doxorubicin induced cardiomyopathy model

Doxorubicin is a commonly used cycle non-specific anti-tumor chemotherapy drug in clinical practice. While anti-tumor, it also forms superoxide free radicals, destroys cell membrane structure and function. Its mechanism of myocardial toxicity is to p...

2024-02-18

[Animal Modeling] - Electrical Stimulation Induced Ventricular Arrhythmia Model

Electrical stimulation can change the membrane conductivity of myocardial cells, increase the heterogeneity of myocardial repolarization, and make the stimulation more prone to fibrillation during the vulnerable period. The ventricular fibrillation t...

2024-02-18

[Animal Modeling] - Acute Myocardial Ischemia and Atrial Fibrillation Model

Acute ischemia delays the conduction of impulses in the ischemic area of the atrium, making it easier for reentry to form and leading to the occurrence of atrial fibrillation...

2024-02-18

【Animal Modeling】-Influence of inflammatory factor storm on immune response to infection in aged mice

After highly pathogenic avian influenza-infected mice, over-expressed inflammatory factors, known as inflammatory factor storm, were detected in the lungs....

2024-02-18