[Animal modeling - Drug efficacy evaluation] - Bacterial bile duct infection model

Guinea pigs weighing 300-400g were anesthetized with ether, and the abdominal cavity was opened to expose the gallbladder. After suctioning out the bile with a 1ml syringe, 3 × 10000 Escherichia coli, or a filtered 20% fresh suspension of guinea pig ...

2024-08-22

[Animal Modeling - Pharmacological Evaluation] - Acute Liver Ischemia Induced Liver Failure Animal Model

Adult animals, anesthetized and fixed, open the abdomen along the midline, and perform a purse string suture on the inferior vena cava above the renal vein using 5-0 vascular suture thread. After finding the portal vein from the root of the mesentery...

2024-08-22

[Animal Modeling - Pharmacological Evaluation] - Liver Resection Induced Liver Failure Animal Model

Adult rats were anesthetized by intraperitoneal injection of ether or ketamine at a dose of 100mg/kg body weight, fixed in a supine position, and opened along a transverse incision in the upper abdomen. The abdominal wall blood vessels were cauterize...

2024-08-22

[Animal modeling - Drug efficacy evaluation] - Induction of liver cirrhosis model by common bile duct ligation method

Adult rats were anesthetized and underwent laparotomy through a midline incision on the navel. The common bile duct was sutured with 7-0 silk thread 1cm from the porta hepatis. After the surgery, regular feeding for 2-8 weeks. During this period, pay...

2024-08-22

[Animal Modeling - Pharmacological Evaluation] - Chemical Induced Cirrhosis Animal Model

Adult male rats were intraperitoneally injected with 0.5% dimethylnitrosamine (DMNA) at a dose of 10mg/kg body weight for 3 consecutive days per week, once a day, for a total of 4 weeks. Alternatively, thioacetamide (TAA) can be intraperitoneally inj...

2024-08-22

[Animal Modeling - Pharmacological Evaluation] - Ethanol Induced Cirrhosis Model

Adult baboons were fed with Liebert Decarh ethanol liquid feed, which consisted of a formula of 18.6% casein, 0.23% L-cysteine, 0.14% DL methionine, 2.3% corn oil, 7.7% olive oil, 0.72% linoleic acid and ethyl linoleate, 70.3% dextrin and maltose sug...

2024-08-22

[Animal Modeling - Pharmacological Evaluation]- Animal Model of Nutritional Insufficiency Cirrhosis

Male weaned rats were fed 8-10g of low protein high-fat feed lacking choline daily for 2-3 months. This feed is composed of casein or soybean as the protein source, supplemented with sucrose, lard, vitamin powder, salt, and a quantitative amount of L...

2024-08-22

[Animal Modeling - Efficacy Evaluation] - Carbon Tetrachloride Induced Cirrhosis Animal Model

Adult male rats were intraperitoneally injected with 2ml/kg body weight of 30% CCl4 paraffin oil solution twice a week for 7 consecutive weeks. Or subcutaneous injection of 3ml/kg body weight of 40% CCL4 soybean oil solution, with the first dose of 5...

2024-08-22

【Animal modeling - Drug efficacy evaluation】 - Induction of liver fibrosis model by common bile duct ligation method

Adult rats were anesthetized and their abdominal cavity was opened to locate and free the common bile duct in the mesentery of the descending duodenum. Two silk threads were threaded underneath and a \"V\" - shaped incision was made towards...

2024-08-22

[Animal Modeling - Pharmacological Evaluation] - Fetal Mouse Colonic Implantation Induced Ulcerative Colitis Animal Model

Adult SD rats, aged 5-6 months and weighing 250-400g, were implanted with a colon of 3-4cm under the right renal capsule of 14-16-day-old homologous fetal rats. On postoperative days 7, 14, and 21, 5-10ml of cardiac blood was extracted for the separa...

2024-08-21

[Animal Modeling - Pharmacological Evaluation] - Stress Induced Duodenal Ulcer Animal Model

Adult rats were fasted without water for 16 hours, anesthetized with intraperitoneal injection of pentobarbital sodium, and fixed. Then, an electric pusher was used to push the hair on the area slightly larger than the intended burn area. Dip a gauze...

2024-08-21

[Animal modeling - Drug efficacy evaluation] - Hot water scald induced stress-induced gastric ulcer animal model

Adult experimental rats were fasted and deprived of water for 24 hours. They were anesthetized by intraperitoneal injection of pentobarbital sodium at a dose of 30mg/kg body weight. The animals were immersed in a hot water tank at 80-92 ℃ for 10-18 s...

2024-08-21

[Animal modeling - Drug efficacy evaluation] - Water immersion restraint method induced stress-induced gastric ulcer animal model

Adult rats were fasted without water for 24 hours, anesthetized with ether, and placed in a supine position. The animal\'s limbs and neck were tied and fixed onto a mouse plate...

2024-08-21

[Animal modeling drug efficacy evaluation] - HP induced chronic atrophic gastritis animal model

Mouse model: BALB/c mice, SPF grade, 6-8 weeks old. After fasting for 12 hours and water deprivation for 8 hours, inoculate Hp (SS1) culture suspension at 1000000000000 CFU/L once every other day, for a total of 3 times, with 100 μ l per time. Prepar...

2024-08-21

[Animal Modeling - Pharmacological Evaluation] - Physical Factors Induced Chronic Atrophic Gastritis Animal Model

Adult rats were orally administered with a 55 ℃ 15% sodium chloride (NaCl) solution or simply 10ml/kg body weight of 55 ℃ distilled water once daily for 12-32 weeks. During this period, the general activity of the animals was continuously observed. A...

2024-08-21

【Animal Modeling - Pharmacological Evaluation 】- Acute Gastritis Animal Model

Adult male rats were fasted without water for 48 hours, orally administered with 100mg/kg body weight of acetylsalicylic acid, or 10mmol/L acetic acid or different concentrations of hydrochloric acid (1, 10, 10mmol/L), or administered with the same b...

2024-08-21