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[Animal modeling - Pharmacodynamic evaluation] - Chronic liver injury model induced by sucrose and alcohol

[Animal modeling - Pharmacodynamic evaluation] - Chronic liver injury model induced by sucrose and alcohol

For the first 3 days, rats were given a 2% sucrose solution for free consumption. Then, 5% alcohol was added to the sucrose solution, and the alcohol concentration was increased by 5% to 15% every 4 days. Afterwards, the alcohol concentration was inc...

2024-07-01
[Animal Modeling Pharmacodynamic Evaluation ]- Animal Model of Congenital Kidney Yang Deficiency Syndrome

[Animal Modeling Pharmacodynamic Evaluation ]- Animal Model of Congenital Kidney Yang Deficiency Syndrome

Adult mice, good at calling ferocious cats. When raising mice to the age of 75 days, they were mated in a ratio of 2:1 male and female. On the evening of the 4th day, male mice were taken out, and on the 5th day, the experimental mice were intimidate...

2024-07-01
[Animal Modeling Pharmacodynamic Evaluation] - Elderly Kidney Yang Deficiency Animal Model

[Animal Modeling Pharmacodynamic Evaluation] - Elderly Kidney Yang Deficiency Animal Model

The content of 3 β - HSD and glucose-6-phosphate dehydrogenase (G-6-PD) in the interstitial cells of the testes of elderly animals, as well as the content of 3 β - HSD in the adrenal cortex, significantly decreased...

2024-07-01
[Animal modeling - Pharmacodynamic evaluation] - Chronic liver injury model induced by total nutrients and alcohol

[Animal modeling - Pharmacodynamic evaluation] - Chronic liver injury model induced by total nutrients and alcohol

Feed rats with liquid feed containing total nutrients and alcohol. This food is formulated based on calories, with 21% fat, 18% protein, 47% carbohydrates (36% of which are replaced by alcohol), as well as some vitamins and inorganic salts. Solid foo...

2024-07-01
[Animal Modeling Pharmacodynamic Evaluation] - Pyrazole Plus Wine Refined Chronic Liver Injury Model

[Animal Modeling Pharmacodynamic Evaluation] - Pyrazole Plus Wine Refined Chronic Liver Injury Model

Rats were fed liquid feed containing total nutrients and alcohol with pyrazole for 12 weeks...

2024-07-01
Animal modeling drug efficacy evaluation - tetracycline induced acute liver injury model in mice

Animal modeling drug efficacy evaluation - tetracycline induced acute liver injury model in mice

The tetracycline modeling group was prepared into a suspension at a dose of 2250mg/kg, administered orally once at a weight of 0.2ml/10g, and the model was formed after 18 hours; The blank control group was given an equal volume of physiological sali...

2024-07-01
[Animal modeling - Pharmacodynamic evaluation] - Alcohol induced acute liver injury model

[Animal modeling - Pharmacodynamic evaluation] - Alcohol induced acute liver injury model

Use 56 degree Baijiu, intraperitoneal injection, rats 7ml/kg body weight each time (mice 16ml/kg body weight each time), twice a day, for 2 weeks...

2024-07-01
[Animal modeling - Pharmacodynamic evaluation] - A model of acute liver injury induced by concanavalin A

[Animal modeling - Pharmacodynamic evaluation] - A model of acute liver injury induced by concanavalin A

Cona bean protein A can activate the proliferation of CD8+or CD4+cells, increase the release of IFN - γ, which promotes the infiltration of inflammatory cells into the hepatic portal vein area and activates the comprehensive response of M phi functio...

2024-07-01
[Animal Modeling Pharmacodynamic Evaluation ]-Thyroidectomy Animal Model of Kidney Yang Deficiency Syndrome

[Animal Modeling Pharmacodynamic Evaluation ]-Thyroidectomy Animal Model of Kidney Yang Deficiency Syndrome

Adult rats were anesthetized, with skin incision, muscle dissection, bilateral thyroid separation and excision, and muscle and skin suturing. Observe the general condition of rats, test left ventricular function and thyroid function, euthanize animal...

2024-07-01
Animal Modeling Pharmacodynamic Evaluation Sex Hormone Application Method Animal Model of Kidney Yang Deficiency Syndrome

Animal Modeling Pharmacodynamic Evaluation Sex Hormone Application Method Animal Model of Kidney Yang Deficiency Syndrome

Select female rat offspring born at the same time period for selective breeding. On the 8th day of age, female rats were subcutaneously injected with testosterone propionate at a dose of 1.25mg/animal in the neck and back, and weaned on the 22nd day ...

2024-07-01
Animal Modeling Pharmacodynamic Screening Rabbit Pneumonia Model

Animal Modeling Pharmacodynamic Screening Rabbit Pneumonia Model

Escherichia coli is seeded on a nutrient agar slope and stored in a refrigerator at 4 ℃. Before use, it is picked up with an inoculation ring on a sterile operating table and transferred to a regular broth medium. It is incubated at 37 ℃ for 48 hours...

2024-07-01
【Animal Modeling】-Polyethylene oxide improves blood perfusion of chronic ischemic hindlimb in rats

【Animal Modeling】-Polyethylene oxide improves blood perfusion of chronic ischemic hindlimb in rats

To evaluate the effect of polyethylene oxide (PEO) on improving blood perfusion of chronic ischemic hindlimb in rats by contrast echocardiography...

2024-07-01
【Animal modeling】-An easy-to-operate method for the isolation and culture of mouse hepatocytes

【Animal modeling】-An easy-to-operate method for the isolation and culture of mouse hepatocytes

To optimize an easy-to-operate, fast and practical method to obtain mouse hepatocytes on the basis of traditional hepatocyte extraction methods, and to provide an improved experimental method reference for researchers engaged in liver function...

2024-07-01
【Animal modeling】-Changes of IL-6, SOD and MDA in the lung tissue of rats with acute pulmonary edema

【Animal modeling】-Changes of IL-6, SOD and MDA in the lung tissue of rats with acute pulmonary edema

To investigate the changes and significance of malondialdehyde, superoxide dismutase and interleukin-6 in lung tissue of acute hypoxia-induced acute pulmonary edema in rats...

2024-07-01
【Animal modeling】-Establishment and application of a method for detecting the copy number of porcine endogenous retrovirus based on droplet digital PCR technology

【Animal modeling】-Establishment and application of a method for detecting the copy number of porcine endogenous retrovirus based on droplet digital PCR technology

To optimize the detection method of porcine endogenous retrovirus copy number in pig genome by using droplet digital PCR technology, and to establish a detection method for PERV copy number...

2024-07-01
【Animal Modeling】-Diversity of intestinal flora of oriental vole under laboratory and field conditions

【Animal Modeling】-Diversity of intestinal flora of oriental vole under laboratory and field conditions

To investigate the distribution and differences of intestinal flora of oriental voles under laboratory and field conditions...

2024-07-01