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How to prepare the ethanol gavage plus high-fat feeding model?

How to prepare the ethanol gavage plus high-fat feeding model?

Replication method Adult rats were fed with a high-fat diet (1% cholesterol and 5% lard based on a low-fat diet) after normal feeding for 1 week, and 60ml ethanol 1.5ml/kg body weight was added at the same time at the end of the experiment. Feed the ...

2020-08-07
How to prepare an animal model of mechanically expandable IBS?

How to prepare an animal model of mechanically expandable IBS?

Replication method 8 days old Wistar rats, using angioplasty (PTCA) balloon (3.0 mm x 20 mm) for colon stimulation (CI), 1 minute/time, 2 times a day (interval 30 minutes), 1 week The sac pressure was 4 kPa (30 mmHg) (weak stimulation pressure), 6 kP...

2020-08-07
How to prepare an animal model of IBS stimulated by ice water?

How to prepare an animal model of IBS stimulated by ice water?

Replication method Take 2 ml of normal saline orally in a two-week-old rat weighing 120-150 g at 0-4°C once a day for 14 consecutive days...

2020-08-07
How to prepare an animal model of intestinal adhesions?

How to prepare an animal model of intestinal adhesions?

Reproduction method: anesthetize adult rats by intraperitoneal injection of 40 mg/kg body weight of sodium pentobarbital, fast for 12-16 hours without drinking water, fix the back on the operating table, and remove the hair regularly...

2020-08-07
How to prepare an animal model of ulcerative colitis induced by Oxazolone?

How to prepare an animal model of ulcerative colitis induced by Oxazolone?

Breeding method BALB/c mice, 7-8 weeks old, weighing 25-30g, shaved abdominal skin (2cm x 2cm), 0.2ml 3% oxazolone (dissolved in 100% ethanol) skin, once every other day Repeat for 5 days, insert a 2 mm diameter silicone tube through the anus into th...

2020-08-07
How was the animal model of ulcerative colitis induced by dinitrochlorobenzene prepared?

How was the animal model of ulcerative colitis induced by dinitrochlorobenzene prepared?

Replication method In adult male BALB/c mice, cut abdominal hair (without damage to the skin) into approximately 2.0 cm x 1.5 cm and 3.3% 2,4-dinitrochlorobenzene (DNCB) acetone olive oil. Solution (1:1 ratio) abdominal smear, once a day, 50μl a day,...

2020-08-07
How to prepare an animal model of Crohn disease?

How to prepare an animal model of Crohn disease?

Replication method: Adult rats fasted for 48 hours were anesthetized by intraperitoneal injection of sodium pentobarbital, a newborn gastric tube was inserted to a depth of about 8 cm, and trinitrobenzene sulfonic acid (2, 4,6-trinitrobenzene) Benzen...

2020-08-07
How to prepare an animal model of appendicitis?

How to prepare an animal model of appendicitis?

Reproduction method After anesthesia, the adult rat is fixed, the abdomen is opened under aseptic conditions, the intestine connected with the appendix, terminal ileum and appendix mesentery (hereinafter referred to as the adjacent intestine) is take...

2020-08-07
How to prepare an animal model of stress duodenal ulcer?

How to prepare an animal model of stress duodenal ulcer?

Breeding method Adult rats fasted in water for 16 hours were anesthetized by intraperitoneal injection of sodium pentobarbital, fixed, and then covered with electric clippers to cover a slightly wider area than the burned site...

2020-08-07
How to prepare animal model of chronic gastric ulcer induced by acetic acid cautery method?

How to prepare animal model of chronic gastric ulcer induced by acetic acid cautery method?

Reproduction method Anesthetize adult rats with sodium pentobarbital, incise the abdomen in the supine position, and inject 0.01 to 0.05ml of 20% to 100% acetic acid into the anterior wall of the gastric antrum with a 0.01ml tuberculin syringe...

2020-08-07
How to prepare an animal model of acute gastric ulcer induced by pyloric ligation?

How to prepare an animal model of acute gastric ulcer induced by pyloric ligation?

Replication method Adult rats, fasted and fed water for 48 hours, anesthetized with ether, incised the abdominal wall to expose the stomach, pull the stomach out of the abdominal cavity with blunt forceps, and suture the junction of the pylorus and d...

2020-08-07
How to prepare an animal model of acute gastric ulcer induced by chemical substances?

How to prepare an animal model of acute gastric ulcer induced by chemical substances?

Reproduction method: adult experimental mice, fasting for 48 hours, oral gavage of ethanol (5 ml/kg body weight) or acetylsalicylic acid (200 mg/kg body weight) or 0.6 mol/L hydrochloric acid (10 ml/kg body weight) 1 After hours, kill the animal by c...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by immune method?

How to prepare an animal model of chronic atrophic gastritis induced by immune method?

Replication method Adult rats, subcutaneously injected 0.3ml/emulsion, made from the same strain of rat gastric mucosa, which contains the same saline tissue homogenate and 1:1 adjuvant, injected subcutaneously, repeated every 3-4 weeks...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by chemical substances?

How to prepare an animal model of chronic atrophic gastritis induced by chemical substances?

Reproductive method Put 0.05% methylnitrosonitrosoguanidine (MNNG) into a drinking bottle for a 5-6 week old rat, and drink 50 ml per mouse per day for 1 month. Or pour 0.01 to 0.02% ammonia water into a water bottle and drink it for 3 to 6 months fo...

2020-08-07
How to prepare an animal model of chronic atrophic gastritis induced by physical factors?

How to prepare an animal model of chronic atrophic gastritis induced by physical factors?

Reproduction method Adult rats take 10 ml/kg body weight once a day in 15% sodium chloride (NaCl) solution at 55°C or simple distilled water at 55°C for 12 to 32 weeks...

2020-08-07
How to prepare an animal model of acute gastritis?

How to prepare an animal model of acute gastritis?

Replication method Adult male rats, fasted for 48 hours, no water, acetylsalicylic acid 100 mg/kg body weight or 10 mmol/L acetic acid or different concentrations of hydrochloric acid (1, 10, 10 mmol/L) or the same type of bile Or 2 mmol. /L Taurine ...

2020-08-07